FastQCFastQC Report
Wed 22 Jul 2015
G88-M11_GTCCGC_L006_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M11_GTCCGC_L006_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10716311
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT146170.13639955018102778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCA23650.020.6391432
CGATCAC25100.020.372883
CACGATC27000.018.9494021
ATTGGAC103150.015.9983956
GAATTGG127850.013.9983644
AATTGGA127450.013.8234585
GGAATTG141100.012.947893
CTGGAAT187300.012.7393181
AAGACGT59450.012.1202983
CGCGTTT5403.9673978E-812.0587111
CGCAATT14050.011.9177091
GCGTAAT5152.7892747E-711.7409361
AGACGTG63000.011.732054
TTGGACT145050.011.5050897
TAAGGCG22700.011.467739
CGATAAC8950.011.4331591
CGCGTGT8157.2759576E-1211.4140291
CGCCATT24950.011.3717141
ACGTGGT65500.011.355236
GACGTGG65100.011.2821895
Produced by FastQC (version 0.11.2)