FastQCFastQC Report
Wed 22 Jul 2015
G88-M12_GTGAAA_L006_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M12_GTGAAA_L006_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11733980
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT148550.1265981363527124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC26500.021.9273533
ACGATCA28750.019.5645352
CACGATC32800.018.293511
ATTGGAC106200.016.895466
GAATCGC16150.016.4068183
AATTGGA131750.014.6068635
GAATTGG135700.014.5926834
CGCAATT15600.014.3119191
GGAATTG145400.013.9713723
CGCAAAT8500.013.1332881
CTGGAAT199450.012.9897971
GGTAATC31200.012.9624392
CTCGAAT31650.012.3448771
CGTTAAC5706.4046617E-912.2404581
TTGGACT150100.012.1398317
AATCGCA23100.012.0738194
AGAATCG22050.011.8059652
AGTCTAA76150.011.7202955
GTCTAAA79250.011.6724236
CCGGGAT25250.011.6054071
Produced by FastQC (version 0.11.2)