FastQCFastQC Report
Wed 22 Jul 2015
G88-M12_GTGAAA_L006_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M12_GTGAAA_L006_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11733980
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT137220.11694241851443415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCA23600.022.0377922
CGATCAC24500.021.4483223
CACGATC29750.018.6508271
ATTGGAC99350.015.5216336
GAATTGG126850.013.2019954
CGCGGAT11650.013.1826531
CTCGAAT26700.013.1738931
CGCCGTT6407.6397555E-1113.1593971
AATTGGA124800.013.14339355
GAATCGC16500.013.0961723
GGAATTG141250.012.4123913
CTGGAAT193800.012.2958441
GTCGATT10100.012.2626891
AGTCTAA75900.012.035025
CGCCATT27600.011.4878291
GTCTAAA81100.011.3844656
CGGGATT19750.011.2878831
CGATTGG93800.011.04727654
CGGCGAT8557.094059E-1111.0091691
AGATCGG97000.010.97101292-93
Produced by FastQC (version 0.11.2)