FastQCFastQC Report
Wed 22 Jul 2015
G88-M2_TGACCA_L006_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M2_TGACCA_L006_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15585424
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT196730.12622691561038057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCA37100.024.0578162
CGATCAC39050.023.5707323
CACGATC42600.021.6207921
ATTGGAC149800.015.0497596
GAATTGG188650.013.2071054
AATTGGA184950.013.0440565
CGCGTGT11450.012.5942461
GGAATTG206000.012.2761043
CTGGAAT270100.012.072841
AAGACGT89000.012.0656543
AGACGTG95400.011.5478094
TTGGACT199950.011.4610327
GAATCGC23800.011.3286963
AGTCTAA113600.011.0480365
CTCGAAT38700.010.9382151
GACGTGG97200.010.9035585
GGTAATC45850.010.8485912
AAAGACG104200.010.8409522
CGATAAC14600.010.8328331
GTCTAAA116750.010.7897656
Produced by FastQC (version 0.11.2)