FastQCFastQC Report
Wed 22 Jul 2015
G88-M2_TGACCA_L006_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M2_TGACCA_L006_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15585424
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATT188500.12094634063211883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC36550.024.0585983
ACGATCA37750.023.230842
CACGATC42200.021.9601841
ATTGGAC144400.014.8668496
AAGACGT81350.013.4060283
GAATTGG178000.013.1368634
GGAATTG199750.012.9115143
AATTGGA178350.012.8356395
CTGGAAT256450.012.8120221
CGCCATT33950.012.5535191
AGACGTG89600.012.2813114
CGATTGG138100.011.9166954
AAAGACG96650.011.8418262
GGTAATC44200.011.7699772
GCGATTG133150.011.7693973
AGTCTAA118250.011.6321935
CCGGAAT46450.011.629141
GTCTAAA119500.011.5108747
GACGTGG90700.011.374095
ACGTGGT92400.011.3243256
Produced by FastQC (version 0.11.2)