FastQCFastQC Report
Wed 22 Jul 2015
G88-M3_ACAGTG_L006_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M3_ACAGTG_L006_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13032720
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA2601.4559423E-414.3122081
CTCGATT11650.013.9743671
CGCGTAT2400.001291904713.5667791
GAATCGC21200.013.1569043
ATTGGAC64200.012.5985116
CGCGGAT13400.011.4551071
CGATCAC21800.011.3020623
AGATCGG96200.010.77692392-93
AATTGGA91900.010.7232245
CGTAAAT8251.1823431E-1010.7124711
AATCGTG16250.010.5840855
CGGGGAT30900.010.5373061
CTCGAAT33000.010.4305641
CCGGAAT42550.010.3851811
GAATTGG96550.010.2067764
AATCGCA31050.010.1801064
GATCGGA85600.010.15623892-93
CTGGAAT186250.010.06465151
ATCGCAC19400.010.0635825
TAAGGCG38350.010.0604199
Produced by FastQC (version 0.11.2)