FastQCFastQC Report
Wed 22 Jul 2015
G88-M5_CAGATC_L006_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M5_CAGATC_L006_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15092239
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT3851.26262585E-512.8642991
CCGAAAT23700.011.0757811
CGATCAC25100.010.7579633
CGCCGAT9857.2759576E-1210.5591741
CTAGCCT71700.010.29233551
AGATCGG103450.010.27919392-93
CGCAAAT15050.010.2016881
TAGCCTC69000.010.1900622
CTGGAAT204750.010.1836861
CTCGCGT12850.010.0211381
GTCGATT14350.010.0090511
GTTCGAT23800.09.98874951
CGACGAT9951.09139364E-109.9552861
CTCGTTT33400.09.9351671
AATCGTG17300.09.9326015
CTCGTCT65450.09.9130771
GAATCGC23800.09.90163
CGGGAAT31150.09.8578111
ATTGGAC62000.09.8190866
CGCGGAT14200.09.7659961
Produced by FastQC (version 0.11.2)