FastQCFastQC Report
Wed 22 Jul 2015
G88-M6_CTTGTA_L006_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M6_CTTGTA_L006_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17039792
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAC16500.012.1230921
CTAGCCT78850.011.7993251
CGCGTGT12250.011.7721141
TAAGGCG63600.011.7673579
GAATCGC26000.011.2641333
CGCAAAT12100.011.1491421
TAGCCTC76900.010.7603272
ATTGGAC68450.010.66194256
CTCGAAT47600.010.6524241
CCGGAAT51050.09.9325241
CCGGCAT37400.09.8261721
AGATCGG130100.09.77318492-93
GATCGGA112150.09.7000292-93
CCGGGAT36500.09.6861141
CTGGAAT221550.09.6796151
CCCGAAT38500.09.54542351
AATCGCA32750.09.5097944
CTCGGAT62400.09.4677761
CGGAAAT40400.09.32679751
CGGGAAT38100.09.2793481
Produced by FastQC (version 0.11.2)