FastQCFastQC Report
Wed 22 Jul 2015
G88-M6_CTTGTA_L006_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M6_CTTGTA_L006_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17039792
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCT81100.011.3814711
TAAGGCG63750.011.2286169
CCGGAAT54000.010.6603271
CTCGAAT44700.010.5468411
CGACAAT21200.010.533751
GAATCGC24650.010.386533
TAGCCTC80750.010.3224872
CGGAAAT37000.010.1933791
CGCAATT27400.010.142471
CGATTGG54950.010.0353934
AGATCGG127150.09.88315592-93
CGGTTTA18150.09.7658473
GTCGGTT39250.09.735481
CCGGGAT38450.09.6799081
ATTGGAC66700.09.5962516
CTCGGAT62650.09.5845251
CTGGAAT217050.09.579891
GATCGGA110800.09.55077592-93
TCGGTTT40800.09.485322
TAGATCG26500.09.38082492-93
Produced by FastQC (version 0.11.2)