FastQCFastQC Report
Wed 22 Jul 2015
G88-M7_AGTCAA_L006_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M7_AGTCAA_L006_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14486725
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGC23800.014.8441493
CGCGTGT11350.013.5241391
CTAGCCT70200.013.5171341
GTCGATT12550.013.3429041
CGCGATT4657.372546E-813.004151
TAGCCTC68850.012.963312
AATCGCA28350.012.6251144
TAAGGCG67200.012.3818189
AGAATCG32550.011.567852
CGCGCAT8803.6379788E-1211.1001331
CTCGAAT39450.010.9654761
CGCGGAT11750.010.2926451
CGGAAAT31450.09.9093621
CCGGCAT30350.09.8087311
AGATCGG101550.09.7969892-93
CTGGAAT198150.09.7654171
ATCGCAC19650.09.6988895
CCGGGAT29750.09.693851
CGATTCG4802.2734789E-49.6845053
CGGGAAT29400.09.6510391
Produced by FastQC (version 0.11.2)