FastQCFastQC Report
Wed 22 Jul 2015
G88-M7_AGTCAA_L006_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M7_AGTCAA_L006_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14486725
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCCT71600.013.0049161
TAGCCTC67450.012.554112
GAATCGC22000.012.0525593
CTCGAAT41300.011.8726551
TAAGGCG62600.011.8660249
CGATCAC22600.011.732583
GTTCGAT22250.011.35279751
CGGAAAT29750.010.8215421
CGCGTGT10550.010.7978721
CGCAATT22000.010.5812731
CGACGAT11100.010.2628411
CGCAAAT12150.010.1912271
ATTGGAC53700.010.14989956
GTCGATT13200.010.1310071
GATTGGC120250.010.0044695
CCACTAG49600.09.99883
TCGCGTG9951.09139364E-109.9535662
CTGGAAT192250.09.9445171
CTCGTTT29900.09.9390031
AGATCGG99500.09.73263692-93
Produced by FastQC (version 0.11.2)