FastQCFastQC Report
Wed 22 Jul 2015
G88-M8_AGTTCC_L006_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M8_AGTTCC_L006_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15388322
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAC28050.013.7557833
ACGATCA28300.013.4700412
CACGATC36200.013.2339341
ATTGGAC81700.013.14347656
CTAGCCT69150.012.5106831
CGCGTGT11350.012.2937871
CGCGATT5253.579953E-711.5171331
CTCGAAT40950.011.3581181
GAATCGC22950.011.3434573
TAAGGCG64100.011.1681239
GAATTGG121800.010.7245234
CTGGAAT225900.010.3152991
CGCCAAT19900.010.2839481
TAGCCTC69400.010.248812
CGGGAAT31050.010.1860921
AATTGGA114250.010.131265
CGCGAAT14600.09.8757291
CCGGAAT44300.09.8692561
CGCGTCT14650.09.8420231
GGAATTG140400.09.7346493
Produced by FastQC (version 0.11.2)