FastQCFastQC Report
Wed 22 Jul 2015
G88-M8_AGTTCC_L006_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG88-M8_AGTTCC_L006_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15388322
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGC24500.013.031153
ATTGGAC82600.012.5469856
CGATCAC28600.012.5369623
ACGATCA29100.012.4256162
CTAGCCT74600.011.9531671
GTTCGAT21850.011.336211
CGCGTGT11800.011.3352491
CGCGATT4851.0784632E-511.2341182
CACGATC34000.011.2191791
CGATAAC14050.010.9303651
TAGCCTC76450.010.8847862
CTCGTTT30600.010.8468261
AATCGCA29000.010.6703334
TAAGGCG60250.010.58134659
GAATTGG119350.010.5766014
CGCGTAT3300.00873387810.5083231
CTGGAAT219150.010.48881
CGCCATT36000.010.4582821
AATTGGA115900.010.2134065
GGAATTG139350.010.1865313
Produced by FastQC (version 0.11.2)