FastQCFastQC Report
Sun 26 Mar 2017
130524Wax_D13-2448_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130524Wax_D13-2448_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences21858067
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACGGTGATCTCGTA280910.12851548126373663Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACG34450.039.01629333
CGACGGT32550.038.65788335
GATCGGA52800.036.207931
CCGACGG37500.035.6669834
ATGCCGA52500.035.19711325
TCTCGTA45700.035.0921844
GACCGAC39450.034.46286832
GAGCGGT55850.034.2196359
ATCGGAA56650.033.928872
CGGTTCA56800.033.53037612
AGCGGTT57050.033.38420510
GATCTCG47150.033.2616142
CGAGACC50600.032.8207729
GCGGTTC58500.032.44320711
CCGAGAC53250.032.38995728
ATCTCGT50250.032.21795743
AATGCCG58300.032.1843524
AGACCGA43000.031.87538131
TGCCGAG58050.031.83398226
CGGTGAT40850.031.65701738
Produced by FastQC (version 0.11.2)