FastQCFastQC Report
Sun 26 Mar 2017
130524Wax_D13-2450_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130524Wax_D13-2450_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences27365605
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA36150.030.5482541
TCTCGTA31500.029.88884444
GACCGGT24800.029.1830932
ACCGGTA24950.028.65480633
CGGTAAC25550.028.41228135
CGGTTCA41600.028.07121712
ATGCCGA40000.027.88240625
GAGCGGT42950.027.5481649
ATCGGAA43200.027.2343752
AGCGGTT43750.026.84335110
CCGGTAA27350.026.54241634
GCGGTTC44350.026.4794211
AATCTCG32150.025.0387942
CGAGACC38950.024.73466529
AATGCCG45900.024.72818824
ATCTCGT38950.024.00026943
TGCCGAG46900.023.68710126
CCGAGAC41550.023.66627328
GAGACCG40200.023.36799230
AAGAGCG51700.023.0977757
Produced by FastQC (version 0.11.2)