FastQCFastQC Report
Sun 26 Mar 2017
G91_M1M2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG91_M1M2.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences44575595
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT36600.036.6636535
ACCGACG40150.035.34007633
ATGCCGA91500.034.95637525
GATCGGA92600.034.8975831
TCTCGTA80150.034.36204544
CGGTTCA97000.033.3059312
ATCGGAA101600.032.684152
GAGCGGT102000.032.0408679
CCGACGG44300.032.02929334
AGCGGTT102200.031.95664610
AATGCCG102400.031.426824
CGAGACC92500.031.1511229
GCGGTTC104550.031.1321911
CCGAGAC94150.031.05294628
ATCTCGT91100.030.32499343
TGCCGAG104400.030.32204426
GAGACCG95350.029.97221230
AAGAGCG112650.029.4400277
GCCGAGA108000.028.86318827
TCGGAAG117450.028.367013
Produced by FastQC (version 0.11.2)