FastQCFastQC Report
Sun 26 Mar 2017
G91_M3M4.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG91_M3M4.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences55319193
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCATCACATCTCGTA842980.15238472477355192Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA150500.037.12624744
ATGCCGA170650.036.63622325
GATCGGA174400.036.1373671
CGCATCA111350.035.77764535
ACCGCAT113750.035.31327433
AGCGGTT183400.035.14705310
CGGTTCA182450.035.13636812
AGACCGC133500.035.11802331
CGAGACC163700.035.0426429
ATCGGAA185750.035.0316242
ATCTCGT161400.034.73767543
GAGCGGT187000.034.5292249
CCGAGAC168650.034.2802928
GACCGCA118250.034.09995732
AATGCCG185300.034.0695224
GCGGTTC189450.033.93100711
TGCCGAG185500.033.71618726
GAGACCG170050.033.44350430
CCGCATC120000.033.1989734
AAGAGCG200550.032.458127
Produced by FastQC (version 0.11.2)