FastQCFastQC Report
Tue 11 Oct 2016
130524Wax_D13-2451_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130524Wax_D13-2451_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences20162882
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA27650.032.943751
TCTCGTA25750.031.18314444
CGGTTCA30200.029.71469912
ATGCCGA30200.029.13783825
TATCTCG28450.028.84078642
ATCGGAA32300.028.5309092
GAGCGGT32000.028.2499399
AGCGGTT32150.027.98095710
CGGGATC29950.027.61470235
ATCTCGT29100.027.29053143
CGAGACC31800.026.35658629
CCGAGAC31900.026.34560628
GCGGTTC34150.026.34218411
CCGGGAT31450.026.2979534
AGACCGG32050.025.80749131
AATGCCG34900.025.5911524
GACCGGG32750.025.18833432
TGCCGAG35300.025.17883926
ACCGGGA33200.025.17784533
AAGAGCG36750.025.1365537
Produced by FastQC (version 0.11.2)