FastQCFastQC Report
Tue 11 Oct 2016
130524Wax_D13-2452_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130524Wax_D13-2452_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences22325991
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCG10150.023.62169334
CGTCCGG10400.023.47644835
GATCGGA13200.023.0007651
ATCGGAA15150.020.1789782
GACCGTC11950.020.06518632
TCTCGTA11900.019.7803144
CGGTTCA15400.019.70911212
GAGCGGT16000.018.9702329
ACCGTCC12950.018.85472933
CGAGACC14400.018.33250429
GCGGTTC17550.017.41996811
GTCCGGG14700.016.90852736
ATGCCGA17050.016.90268925
AGCGGTT18400.016.8543110
CCGAGAC16800.016.10818328
GAGACCG17100.015.82409530
AGACCGT15900.015.49587631
AAGAGCG19900.015.2521427
CGGGATC14750.014.91167439
TGCCGAG20050.014.81375926
Produced by FastQC (version 0.11.2)