FastQCFastQC Report
Tue 11 Oct 2016
130524Wax_D13-2454_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130524Wax_D13-2454_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences25096174
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTTA17250.026.52357734
CGCTTAA17350.026.11672235
TCTCGTA19100.023.84212944
GATCGGA22350.023.7243961
ACCGCTT19800.022.55277433
GACCGCT20200.022.54240232
CGGTTCA23850.022.13267912
ATGCCGA23350.021.66907325
AGACCGC20950.021.63086931
ATCGGAA25600.021.392562
GAGCGGT24900.021.0230039
AGCGGTT24700.020.83687610
AATCTCG21900.020.59168242
GCGGTTC26400.019.99494211
CGAGACC23700.019.77089529
ATCTCGT23800.019.13354343
AATGCCG28400.018.047524
AAGAGCG29750.017.9651327
CCGAGAC26300.017.81777828
TCGGAAG31100.017.750893
Produced by FastQC (version 0.11.2)