Basic Statistics
Measure | Value |
---|---|
Filename | 130524Wax_D13-2455_NA_sequence.fastq |
File type | Conventional base calls |
Encoding | Illumina 1.5 |
Total Sequences | 21418624 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCGTTATATCTCGTA | 60190 | 0.2810171185599971 | Illumina Paired End PCR Primer 2 (97% over 36bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGCGTTA | 7180 | 0.0 | 41.572487 | 35 |
ACCGCGT | 7350 | 0.0 | 40.462917 | 33 |
GCGTTAT | 7430 | 0.0 | 40.20357 | 36 |
TCTCGTA | 7410 | 0.0 | 40.198875 | 44 |
CGTTATA | 7485 | 0.0 | 39.99426 | 37 |
TATCTCG | 7700 | 0.0 | 38.99635 | 42 |
ATGCCGA | 8635 | 0.0 | 38.444054 | 25 |
GACCGCG | 7875 | 0.0 | 38.10174 | 32 |
CCGCGTT | 7875 | 0.0 | 38.07212 | 34 |
ATCTCGT | 7905 | 0.0 | 37.62514 | 43 |
AGACCGC | 8015 | 0.0 | 37.35448 | 31 |
AATGCCG | 9245 | 0.0 | 36.23873 | 24 |
CGAGACC | 8350 | 0.0 | 36.172394 | 29 |
GAGACCG | 8375 | 0.0 | 36.11737 | 30 |
CCGAGAC | 8385 | 0.0 | 35.998535 | 28 |
GATCGGA | 9470 | 0.0 | 35.9645 | 1 |
TGCCGAG | 9310 | 0.0 | 35.73007 | 26 |
ATCGGAA | 9665 | 0.0 | 35.454266 | 2 |
CGGTTCA | 9730 | 0.0 | 34.72045 | 12 |
GAGCGGT | 9770 | 0.0 | 34.60146 | 9 |