FastQCFastQC Report
Tue 11 Oct 2016
130524Wax_D13-2456_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130524Wax_D13-2456_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences22546648
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCAGCGATCTCGTA718280.3185750715583088Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA90400.039.300844
GCGATCT91950.039.18231640
ATGCCGA114200.038.58374825
AGCGATC94000.038.49091739
CGATCTC93850.038.41250641
AATGCCG116650.037.9606124
GATCTCG98100.037.82826242
GATCGGA121450.037.335511
ATCTCGT94400.037.12249843
CGAGACC107400.037.1138329
CAGCGAT99300.036.50321238
AGACCGG109300.036.3680431
ATCGGAA124650.036.3649372
TGCCGAG121550.036.19906626
CCGAGAC110450.036.17247828
GAGACCG110350.036.06249630
CGGTTCA124000.035.97104312
AGCGGTT124800.035.8465210
GACCGGC111550.035.69316532
GAGCGGT126750.035.3822679
Produced by FastQC (version 0.11.2)