FastQCFastQC Report
Tue 11 Oct 2016
130524Wax_D13-2457_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130524Wax_D13-2457_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences23497206
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTACCGCATCTCGTA800300.34059368590461353Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCG89350.041.95020335
GACCGTA91800.040.97673832
ACCGTAC92250.040.91940333
TCTCGTA92850.040.4438244
CCGTACC93700.040.21429434
GTACCGC94200.039.69693836
TACCGCA94900.039.49465637
ACCGCAT97700.039.26330638
AGACCGT98550.038.1706831
CGCATCT100700.037.983940
ATGCCGA112100.037.91421525
ATCTCGT100400.037.6865243
CCGCATC101800.037.6380439
GATCGGA115900.037.302341
CCGAGAC106150.036.97496428
CGAGACC106200.036.95409829
CGGTTCA116600.036.83996612
AATGCCG115950.036.82481424
GAGACCG106650.036.757730
ATCGGAA119650.036.377972