Basic Statistics
Measure | Value |
---|---|
Filename | 130524Wax_D13-2457_NA_sequence.fastq |
File type | Conventional base calls |
Encoding | Illumina 1.5 |
Total Sequences | 23497206 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTACCGCATCTCGTA | 80030 | 0.34059368590461353 | Illumina Paired End PCR Primer 2 (97% over 36bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTACCG | 8935 | 0.0 | 41.950203 | 35 |
GACCGTA | 9180 | 0.0 | 40.976738 | 32 |
ACCGTAC | 9225 | 0.0 | 40.919403 | 33 |
TCTCGTA | 9285 | 0.0 | 40.44382 | 44 |
CCGTACC | 9370 | 0.0 | 40.214294 | 34 |
GTACCGC | 9420 | 0.0 | 39.696938 | 36 |
TACCGCA | 9490 | 0.0 | 39.494656 | 37 |
ACCGCAT | 9770 | 0.0 | 39.263306 | 38 |
AGACCGT | 9855 | 0.0 | 38.17068 | 31 |
CGCATCT | 10070 | 0.0 | 37.9839 | 40 |
ATGCCGA | 11210 | 0.0 | 37.914215 | 25 |
ATCTCGT | 10040 | 0.0 | 37.68652 | 43 |
CCGCATC | 10180 | 0.0 | 37.63804 | 39 |
GATCGGA | 11590 | 0.0 | 37.30234 | 1 |
CCGAGAC | 10615 | 0.0 | 36.974964 | 28 |
CGAGACC | 10620 | 0.0 | 36.954098 | 29 |
CGGTTCA | 11660 | 0.0 | 36.839966 | 12 |
AATGCCG | 11595 | 0.0 | 36.824814 | 24 |
GAGACCG | 10665 | 0.0 | 36.7577 | 30 |
ATCGGAA | 11965 | 0.0 | 36.37797 | 2 |