FastQCFastQC Report
Tue 11 Oct 2016
130524Wax_D13-2458_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130524Wax_D13-2458_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences23727877
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA20550.029.54679144
GACCGAT20250.029.3304632
GATCGGA23300.027.857161
ACCGATG22600.026.2802833
CGGTTCA25500.025.96181512
CCGATGA23200.025.59996834
CGATGAT23800.025.508635
ATCGGAA26700.024.794732
GAGCGGT26600.024.5577759
AATCTCG24450.024.29209942
ATGCCGA27350.024.21050825
AGCGGTT27550.023.9503110
AGACCGA25900.023.18750431
CGAGACC27600.022.79558829
ATCTCGT27550.022.35842743
GCGGTTC30150.021.95784611
CCGAGAC28250.021.805728
GAGACCG29300.021.17287830
AATGCCG31250.021.04749324
TGCCGAG32200.020.63369226
Produced by FastQC (version 0.11.2)