FastQCFastQC Report
Tue 11 Oct 2016
130524Wax_D13-2459_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130524Wax_D13-2459_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences22986662
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAAGTTATCTCGTA2903501.263123806318638Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA322500.042.7020944
CGCAAGT323950.042.66771735
ACCGCAA327150.042.60930633
AGACCGC325850.042.5651331
GACCGCA327550.042.53142532
ATGCCGA348350.042.47582625
CGAGACC327700.042.43343729
CCGCAAG330100.042.24056634
CCGAGAC328800.042.2289828
GAGACCG329050.042.21291730
GCCGAGA349150.042.03616727
TATCTCG331900.041.93404842
ATCTCGT330100.041.75848443
TGCCGAG353850.041.75707626
AATGCCG356700.041.7023524
GATCGGA370200.040.956441
ATCGGAA374050.040.541572
CGGTTCA370100.040.44056312
GAGCGGT370950.040.3608139
AGCGGTT372400.040.238410