FastQCFastQC Report
Tue 21 May 2019
130524Wax_D13-2460_NA_sequence_40bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130524Wax_D13-2460_NA_sequence_40bp.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences24757647
Sequences flagged as poor quality0
Sequence length40
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGTCC2773131.1201104854593007Illumina Paired End PCR Primer 2 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.7)