FastQCFastQC Report
Tue 11 Oct 2016
130524Wax_D13-2461_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130524Wax_D13-2461_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences19658365
Sequences flagged as poor quality0
Sequence length40
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGGA1315450.6691553443025399Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCA162950.032.8182212
AGCGGTT164850.032.51189810
GAGCGGT166150.032.308599
ATGCCGA162900.032.30728525
GCGGTTC167300.032.00530211
AGACCGT146700.031.97114831
AAGAGCG169250.031.8675547
CGAGACC155450.031.76033229
GACCGTG148500.031.69813332
CCGAGAC156200.031.59437828
GAGACCG156600.031.500730
TGCCGAG167550.031.37008326
GATCGGA172250.031.3451821
ACCGTGG150500.031.26559633
AGAGCGG173200.031.2094028
ATCGGAA173800.031.1355972
AATGCCG170100.031.10982724
GCCGAGA168150.031.00596227
CCGTGGA151800.030.9977634
CGGAAGA175000.030.9191074
Produced by FastQC (version 0.11.2)