FastQCFastQC Report
Tue 11 Oct 2016
130524Wax_D13-2462_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130524Wax_D13-2462_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences23155094
Sequences flagged as poor quality0
Sequence length40
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACTT1031120.44531021985918084Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCA131600.031.7877912
GAGCGGT133250.031.5089139
AGCGGTT133350.031.48528310
ACCGACT119000.031.45425233
ATGCCGA130400.031.37694425
GACCGAC120000.031.34802432
CCGACTT120000.031.1493534
GCGGTTC135450.030.94694311
CGAGACC123500.030.72699229
AGACCGA122000.030.66684731
CCGAGAC123700.030.64624628
GAGACCG124700.030.43780930
AAGAGCG139350.030.3979827
TGCCGAG134550.030.34599726
GATCGGA139400.030.3406521
AATGCCG136100.030.33775124
AGAGCGG141700.029.893858
GCCGAGA137200.029.73553827
ATCGGAA142700.029.664322
CGGAAGA144250.029.366924
Produced by FastQC (version 0.11.2)