FastQCFastQC Report
Tue 11 Oct 2016
130524Wax_D13-2463_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130524Wax_D13-2463_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences21512075
Sequences flagged as poor quality0
Sequence length40
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA21950.026.7294921
GACCGAA20900.024.97934532
GAGCGGT24400.024.313079
CGGTTCA24400.023.82552712
ATGCCGA23800.023.78431725
AGACCGA22650.023.42477231
AGCGGTT24900.023.41525810
ATCGGAA25450.023.0468862
CGAGACC23700.022.74012629
AAGAGCG26900.022.1166827
GAGACCG24500.022.0715530
CCGAGAC25050.021.71624828
GCGGTTC27100.021.38894311
TGCCGAG27200.020.81127726
AATGCCG27550.020.67038724
AGAGCGG29750.020.4550388
CCGAATG25550.020.3000434
GCCGAGA27900.020.22829627
ACCGAAT25700.020.11543533
TCGGAAG29900.019.7283043
Produced by FastQC (version 0.11.2)