FastQCFastQC Report
Tue 11 Oct 2016
130524Wax_D13-2464_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130524Wax_D13-2464_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences23813857
Sequences flagged as poor quality0
Sequence length40
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTAC277950.1167177580683381Illumina Paired End PCR Primer 2 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTA36200.029.54878233
CGGTTCA42400.028.82498412
CCGCTAC37300.028.6317234
ATGCCGA42750.027.87426625
AGCGGTT44200.027.8047710
GATCGGA44800.027.5584771
GCGGTTC44950.027.34084311
GACCGCT39350.027.3130332
GAGCGGT44950.027.2273969
CGAGACC40500.026.99210429
AGACCGC40800.026.38402731
ATCGGAA47600.025.9656472
CCGAGAC42550.025.60949128
AAGAGCG48300.025.5501637
GAGACCG43000.025.23045730
TGCCGAG47550.025.13195626
AATGCCG48800.024.83649424
CGGAAGA51500.024.129134
GCCGAGA49650.023.86374927
TCGGAAG52950.023.4365123
Produced by FastQC (version 0.11.2)