FastQCFastQC Report
Tue 11 Oct 2016
130524Wax_D13-2465_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130524Wax_D13-2465_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences24175674
Sequences flagged as poor quality0
Sequence length40
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCA28500.026.00443312
GATCGGA29350.025.3188231
ATGCCGA29900.024.10512725
AGCGGTT31400.023.7109710
GAGCGGT31350.023.6402979
ATCGGAA32700.022.7716312
GCGGTTC32700.022.7163511
AAGAGCG34100.022.032917
CGAGACC29600.021.94095829
CCGAGAC30700.021.5400928
AGACCGG29500.021.50314131
AATGCCG34650.020.99703624
GAGACCG30800.020.92558130
TGCCGAG34100.020.8370726
ACCGGGC31150.020.1457533
TCGGAAG38100.019.9440163
AGAGCGG38150.019.6493438
GCCGAGA36800.019.53930527
CGGAAGA39400.019.415284
GACCGGG32700.018.982832
Produced by FastQC (version 0.11.2)