FastQCFastQC Report
Tue 11 Oct 2016
130524Wax_D13-2466_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130524Wax_D13-2466_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences21287619
Sequences flagged as poor quality0
Sequence length40
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG11750.027.6439533
CCGTCGT12100.026.70353534
GATCGGA16400.025.8186531
GACCGTC14800.022.29177532
AGCGGTT19400.022.16768610
CGGTTCA19250.022.07572412
GAGCGGT19850.021.4938779
ATCGGAA20950.020.7739322
GCGGTTC21150.020.17274311
ATGCCGA20450.020.0329425
AGACCGT18350.019.3693431
CGAGACC19900.018.88137629
GAGACCG21050.018.5807430
TGCCGAG22600.018.1271126
CCGAGAC21050.017.92855528
AAGAGCG24600.017.7582287
TCGGAAG24950.017.7144993
AATGCCG24350.017.03389224
AGAGCGG27250.016.4055528
CGGAAGA27300.015.8782484
Produced by FastQC (version 0.11.2)