FastQCFastQC Report
Tue 11 Oct 2016
130729Wax_D13-3606_NA_sequence.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filename130729Wax_D13-3606_NA_sequence.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences17830246
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCACTATCTCGTA337600.18934119024493548Illumina Paired End PCR Primer 2 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCAC43200.039.09429635
CGAGACC45000.038.5372929
CCGACCA44300.038.125734
GACCGAC44700.037.9108532
ACCGACC44850.037.85487433
TCTCGTA45400.037.83400744
ATGCCGA48500.037.02194225
GAGACCG47200.036.7410630
AGACCGA47100.036.3524131
GAGCGGT50600.036.2688989
CCGAGAC47700.036.2636728
GATCGGA51000.036.0297551
TATCTCG47850.035.9705742
GCCGAGA50500.035.60224527
CGGTTCA51800.035.34432612
TGCCGAG50750.035.3375726
AGCGGTT52500.034.91493210
GCGGTTC52550.034.8398911
ATCTCGT49700.034.62180343
AGAGCGG54050.034.2758068
Produced by FastQC (version 0.11.2)