FastQCFastQC Report
Wed 29 Jul 2015
G95-M1_CGATGT_L003_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M1_CGATGT_L003_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14729474
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT7950.014.7397631
CGCGAAT7651.8189894E-1212.2542351
CTCGAAT25950.011.9213171
CTCGATT17450.011.8188411
CCGGAAT29350.011.3388211
CTCGCAT18050.010.9066091
CGCCATT29100.010.7919391
CTCGGAT39700.010.6259961
CCGGGAT36250.010.2149611
CCGGCAT29700.09.78482151
CGACAAT14950.09.4058431
CGCCGAT7503.2189928E-79.374491
CCGGTAT16550.09.34616851
CGATCAC20450.09.3224613
CGCCCAT28800.09.1140871
CGCCGTT9953.092282E-98.9505191
CCCGAAT26500.08.8438591
CTCGGTT28650.08.8345981
ACGATCA19900.08.7133082
CGCGGTT10801.4642865E-98.6800831
Produced by FastQC (version 0.11.2)