FastQCFastQC Report
Wed 29 Jul 2015
G95-M1_CGATGT_L003_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M1_CGATGT_L003_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14729474
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT3403.7144622E-513.7040641
CGAATCG4201.4524121E-613.4413013
CGCCGAT8252.0736479E-1011.2954721
CGCGAAT8303.105015E-89.9799351
CTCGAAT23950.09.9434741
CGCGTGT14950.09.6962241
CGATCAC19700.09.6390323
CGCAAAC18300.09.618641
CGAGAAT35000.09.46668151
CTCGCGT10005.840775E-99.3187641
CGGGAAT26300.09.2518371
CCGGAAT34250.09.0693571
CGATTCG4700.0090034178.7355463
CCGCGTT8909.076284E-78.7254351
CGAATGT23350.08.5718723
CCGGCGT16600.08.420571
CGCGGAT9854.305657E-78.4094891
CTGGAAT163750.08.3465781
CGGAGAT31600.08.19161
CGGCGAT12151.86119E-88.0958621
Produced by FastQC (version 0.11.2)