FastQCFastQC Report
Sun 12 Jun 2016
G95-M2_R1_cat.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M2_R1_cat.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12188072
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT6604.116373E-911.3612761
CTCGAAT20700.011.3201121
CGCCTAT13900.010.78912451
CCCGGTT21800.010.5339361
CCGGAAT28250.010.4513681
CCCGAAT21850.010.2953441
CCGGCAT23300.09.6546471
CGACAAT13850.09.47456551
CGCCCTT24550.09.1630661
CGCGGAT7201.8678202E-69.112691
CGCGTAC6202.9397552E-59.0706971
CCCGTAT10407.203198E-109.012551
CGCAAAC16150.08.9958091
AATCGAC11901.4551915E-118.9865985
CGCAATT6808.92941E-68.9595351
CGCGACT4250.0083082538.8216961
CTGCGAT29350.08.7822471
CGGAATC25700.08.7515972
CGAAAAT13401.8189894E-128.743521
CGCCAAT16700.08.6995391
Produced by FastQC (version 0.11.2)