FastQCFastQC Report
Wed 29 Jul 2015
G95-M2_TGACCA_L003_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M2_TGACCA_L003_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12188072
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCATT28800.011.896431
CGCGTAC5008.981795E-611.4205721
CCGATAT12500.010.7976321
CGGAAAT20750.010.257251
CCGGAAT26550.010.1672621
CGGCGAT9854.0563464E-1010.0134231
CGCCGAT5905.583084E-59.6784521
CTGGAAT138950.09.3773561
CGAAGAT26050.09.3660251
CGCAAAT11204.2382453E-109.2699451
CTCGTTT24800.09.2101391
CTCGAAT20900.09.1901071
CGACGAT6251.0480187E-49.1364571
CGATCCG6251.1398675E-49.0662624
CGGGAAT20800.08.9847161
CCCGGAT28200.08.6519491
CGGGTAT11557.185008E-98.5395851
CCGACAT20850.08.4652171
CGATCAC16550.08.3956333
CGCCCAT27900.08.3728531
Produced by FastQC (version 0.11.2)