FastQCFastQC Report
Wed 29 Jul 2015
G95-M3_ACAGTG_L003_R1_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M3_ACAGTG_L003_R1_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17337815
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT10550.011.1085061
CGCGTAT3802.6530118E-411.1026591
CCGGAAT37600.010.8467471
CGCAAAT14950.010.347631
GTCGGAT19550.010.3107391
CCGGGAT42050.010.03331851
CGCCGAT8058.511051E-99.899681
CTCGCAT20600.09.7851921
CTCGGAT46500.09.778831
CGCAATT8904.3419277E-99.4809231
CCGAAAC28000.09.3755791
CGCCAAC30800.09.1320581
CGGGAAT26300.09.090391
CGCGAAT8351.5733895E-78.982591
CTCGATT21450.08.9603431
CGACAAC25250.08.9114421
CCGGAAC45100.08.835081
CTCGAAT28450.08.73296451
CGATCAC26700.08.7071773
CGCGGTT11901.1277734E-108.6665021
Produced by FastQC (version 0.11.2)