FastQCFastQC Report
Wed 29 Jul 2015
G95-M3_ACAGTG_L003_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M3_ACAGTG_L003_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17337815
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT3558.5759937E-411.6010411
CGCAAAT14950.010.3303581
CGACAAT16850.010.0820611
CCGGGAT41700.09.9996391
CGGGAAT32650.09.7756091
CGGCAAT21900.09.402671
CCGGAAT37950.09.2242791
CGCGTGT16350.09.1309411
CGACGAT9603.266541E-89.1161821
CGAAAAT19400.09.0222011
CGGGATT28600.08.8199351
ACGATCA20500.08.7889742
CGCCACT43600.08.6192941
CGCCAAT22900.08.542471
CCCGTAT16400.08.4753031
CGGAAAC34400.08.3804031
CCGGAAC43050.08.370671
CTCGATT22200.08.3480451
CCGCGAT11801.2230885E-88.2890911
CCGAAAT26750.08.27522851
Produced by FastQC (version 0.11.2)