FastQCFastQC Report
Sun 12 Jun 2016
G95-M4_R1_cat.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M4_R1_cat.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14185914
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAT6604.100002E-911.3643831
ACGATCA19000.011.1005732
CGATCAC20500.010.887143
CCGGCAT27200.010.1684071
CTCGAAT22600.010.1638311
CCGGGAT32100.010.0765961
CCGGAAC36350.09.9301571
CCGGAAT28350.09.7559321
CCGATAT15400.09.4364961
CCCGGAT33700.09.3199751
CCGGACT23150.09.3148661
CACGATC28800.09.2779531
CCGCAAT14300.09.1789251
CGCCGTT10356.548362E-109.0585661
CGGGAAT20850.08.9933961
CTCGGTT26850.08.9042161
CTCGAAC41150.08.8857591
CGCCCAT31850.08.8310361
CGGAAAC25300.08.7085761
CGCAATC9156.8623194E-88.7079172
Produced by FastQC (version 0.11.2)