FastQCFastQC Report
Sun 12 Jun 2016
G95-M4_R2_cat.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M4_R2_cat.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14185914
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCAAT14400.010.7750431
ACGATCA20200.010.4974142
CGCATAT10401.0913936E-1110.4435031
CGATCAC22850.010.3195123
CCGGGAT34750.09.5254481
CCGATAT14300.09.4036751
CGGAAAT22650.09.3621571
CCGGAAT31850.09.256051
CGCCACT36600.09.1852831
CTCGAAT21150.09.0479781
CTCGACT21200.09.0266391
CACGATC28250.08.9709391
CGGAGAT30750.08.9143771
CCGAAAT22850.08.8275191
CTCGTAT11801.2150849E-98.7661361
CGCACGT8909.2050686E-78.7168891
CGCGGAT9804.0445957E-78.4441161
TCGATCG6250.00119347188.2017693
CTCGCAT17301.8189894E-128.0719391
AAGACGT33350.07.99272873
Produced by FastQC (version 0.11.2)