FastQCFastQC Report
Sun 12 Jun 2016
G95-M5_R1_cat.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M5_R1_cat.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9627611
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAAT20800.013.5188731
CGCGCAT4608.315492E-712.2257641
CGCGTTT4901.7819857E-611.4772471
CTCGAAT15750.011.3072141
CCCGGAT22300.010.5079431
CTCGAAC22600.09.9537191
CGATCAC13300.09.7892723
TCGGATT10651.0913936E-119.6792972
CGCCATT19500.09.6134211
CGAATTG8251.45137165E-89.581623
CTCGGTT20100.09.5596141
CGACAAC13900.09.4405171
CTCAGAT88050.09.2080851
CTCGGAT26200.09.122661
CGATTGA6657.4360123E-69.0898974
GTCGGAT8251.3129466E-79.0890521
CGGAAAT15600.09.0125821
CTCCGTT21100.08.8844411
CCCGTAT8602.4805377E-78.719151
TCGAACT22000.08.51938152
Produced by FastQC (version 0.11.2)