FastQCFastQC Report
Wed 29 Jul 2015
G95-M6_CTTGTA_L003_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M6_CTTGTA_L003_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15725316
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAAT35750.012.0326991
CGCAAAT13250.09.778791
CCCGAAT26600.09.5471881
CGGAATA19850.09.3989852
CGAGAAT41400.08.7631191
CGCCAAT20400.08.6379321
CTGGAAT171800.08.4770391
CGCCATT36200.08.4470381
CGCAAAC19750.08.3973821
CGACAAT15605.456968E-128.3057041
AAATCGA26450.08.1632214
CTCGAAC41450.08.1273661
CGGCGAT11556.678238E-88.0770261
CACGATC29550.08.0679161
AAGACGT32750.07.99815233
CTCGATT17501.8189894E-127.99625731
CGATCAC21200.07.99482353
GTCGAAT15001.9645086E-107.94689751
ACGATCA19650.07.91220762
CCGAAAC27600.07.88680741
Produced by FastQC (version 0.11.2)