FastQCFastQC Report
Sun 12 Jun 2016
G95-M6_R1_cat.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M6_R1_cat.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15725316
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCAT28650.010.7984681
CCGAAAC25850.09.973441
CGAATCG5204.6764857E-59.8361813
CGCGCAT9651.72804E-109.715061
CGCCCAT32100.09.6378851
CGGCAAT18900.09.4246371
CCGGGAT36150.09.0767971
ACGATCA21800.09.0289692
CGGGTAT13650.08.9286041
CGCGATC8401.7315324E-78.92662
CGGGAAT25800.08.9026481
CGCAATA6859.798765E-68.8940212
CGACAAT14350.08.8197171
CGCAACT14950.08.7792951
CGCGGAT9651.7382263E-88.7435541
CCGGAAT35750.08.6538771
CGCGTGT14650.08.639111
CGCCAAT21200.08.6232621
CTCGTTT32100.08.6156851
CCCGAAT25500.08.4559131