FastQCFastQC Report
Sun 12 Jun 2016
G95-M7_R1_cat.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M7_R1_cat.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20492793
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT3052.4920155E-1019.979971
CGCGATT6251.2914825E-1012.7502961
CTCGAAT32850.010.8451
CTCGCAT27500.010.5684271
CGCCGAT10700.010.5142621
CGCCAAT28700.09.7998791
CGGAATA23150.09.5146192
CGCGCAT12350.09.4890871
CCGGCAT37600.09.3502841
CGCGAAT9207.679773E-99.1714081
CCGGGAT49150.09.1558591
CGCGTTT9752.1427695E-99.1348281
CGCCCTT41100.09.1242991
CGCCCAT43650.09.0208281
CCGGTCT41650.08.8912631
CTCGGTT41700.08.8806031
CGGGAAT32350.08.8390771
CCCGAAT38900.08.7967991
CGCGACT8552.261877E-78.7721331
TCGAATT27900.08.7346122
Produced by FastQC (version 0.11.2)