FastQCFastQC Report
Sun 12 Jun 2016
G95-M7_R2_cat.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M7_R2_cat.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20492793
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAT25200.09.0198361
CGCCAAT25800.09.0103031
CCGGAAT46750.08.84005451
CTCGTAT15801.8189894E-128.5008591
ATCGAGC24150.08.4933246
CGGGTAT19750.08.3700771
CCGGACT34300.08.1329151
AATTCGA27750.08.1298324
CGCACAT34350.08.1210771
CTCGAAT31050.07.9859431
CGATCTA12252.555862E-87.95260434
CGGGAAT33550.07.8527941
CGCAAAT18003.6379788E-127.74886041
CGGGGAT51700.07.6939041
CGCCGAT10902.0577136E-67.58298251
CCGAGAT47950.07.541471
CGCATTT26750.07.5316021
CTGGAAT229250.07.48127031
CGCCCAT45300.07.4124491
CCGGATT32200.07.37986661
Produced by FastQC (version 0.11.2)