FastQCFastQC Report
Sun 12 Jun 2016
G95-M8_R1_cat.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M8_R1_cat.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15500425
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT4903.6379788E-1115.3049681
CGCGTAT2350.001040261713.9617131
CCGGGAT40350.011.2677371
CGCGAAT7703.5470293E-1010.9569651
CTCGGAT35250.010.3715571
ACGATCA18600.010.3296312
CGCGTTT7503.0135197E-89.9992451
CCGGCAT30450.09.8514741
CGCGCAT9055.5661076E-109.8404181
CCCGAAT26900.09.7576281
CGTATGC25500.09.754127546-47
CCCGCAT30500.09.6816461
CCGGTAT17450.09.6697581
CCGGCTT33750.09.5826111
CCGGAAT35700.09.4530681
CTCGTAT25250.09.39066244-45
CTCGAAT24150.09.3160681
CTCGCAT21150.09.3078081
CGCGAGT9601.6225385E-99.2766441
CCGCAAT15700.09.2548751
Produced by FastQC (version 0.11.2)