FastQCFastQC Report
Sun 12 Jun 2016
G95-M8_R2_cat.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M8_R2_cat.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15500425
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTT12850.09.6926581
CGGCAAT18350.09.3328141
CGGGAAT26600.09.1696131
CCCGTAT14005.456968E-128.8964751
CGCCAGT41450.08.8893211
CGCGTAC5855.474692E-48.8711291
CCGGCAT31650.08.6903431
CGCCAAT21700.08.6094931
CGCGTTT9101.2066594E-68.5543031
CCCGGAT35650.08.2975541
CCGAAAC26550.08.2095541
CGCCCAT33800.08.1375551
CTCGAAT21800.08.0938881
CGCAAAT12855.638867E-98.0772151
CCGGAAT39650.07.98401641
CGACGTA7204.8576188E-47.9285721
CTCGATT15102.2009772E-107.90470551
CGCCACT40750.07.8958491
CCGAAAT31000.07.86811971
CCGCAAT15857.4578566E-117.8580851
Produced by FastQC (version 0.11.2)