FastQCFastQC Report
Sun 12 Jun 2016
G95-M9_R1_cat.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M9_R1_cat.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13063534
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT7350.013.3906081
CGGCAAT15050.010.8993311
CCGGAAT30750.010.8213521
CGCCCAT29500.010.1677831
CCCGAAT24900.09.9757341
GCGGGTT11950.09.8048381
CCGGCAT27300.09.7854431
CCGGGAT31700.09.7578241
CGCAAAT10154.3655746E-119.6966471
CGCGGAT8753.252353E-99.6412371
CCGGTTT20250.09.2577041
CTCGAAT21300.09.2414051
CTCGGTT27100.09.1658951
CCGCAAT15400.09.1299591
CGATCTA6152.9334078E-59.0723754
CGACAAT13101.8189894E-128.9441081
CCCGTAT10601.031367E-98.8428541
CTCGAAC34600.08.8045181
CGCGGTT10305.7643774E-98.6453911
CGCACGT11404.4383341E-108.6334181
Produced by FastQC (version 0.11.2)