FastQCFastQC Report
Fri 10 Jun 2016
G95-M1_CGATGT_L002_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG95-M1_CGATGT_L002_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7292425
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAT5351.1312295E-813.1923041
CGGGTAT6851.7644197E-1012.5113661
CGGCATT13300.011.7505031
CGCAAAT6108.7812987E-710.7438491
CTCGATT8153.1559466E-910.5156881
CGCCATT15650.09.986051
CCGATAT7105.7076795E-79.9406791
CACGATC14250.09.90581
CCGGGAT17350.09.87929251
CTCGGTT14900.09.8120131
CGGGAAT11851.8189894E-129.7848881
CCGGAAT15150.09.6500991
CGATCAC10902.6011548E-109.5119113
GTCCGAT8053.123041E-79.3938091
GAATCGA9001.0277836E-79.2349434
CGTCGAT4950.00154514459.1660811
GTCGATT6101.10643814E-49.0909491
CTGGAAT86150.09.0703241
GTTCGAC5150.00221541138.8101161
GCGGTAT6351.7120657E-48.7330371
Produced by FastQC (version 0.11.2)